Next has confirmed its store in the Queensmere Observatory Shopping Centre will close in the autumn.
The retailer said the shop is to shut as the store is at the end of its lease and discussions are currently being held with its staff.
It also said the Maidenhead branch will be closing and ‘both areas will be serviced by our store on the Bath Road Retail Park'.
Marks & Spencer announced it will also be quitting Slough town centre at the end of the month, with BHS, Topshop and River Island leaving last year.
Simon Hall, economic growth and enterprise manager for Slough Borough Council, said he is very disappointed to see Next go but understands the pressures on retailers in recent years.
He said the losses present an opportunity for new and different things to come to the town, with the council looking at places like Camden in London as inspiration to offer pop-up shops and other ideas they may not have had the opportunity to explore in the past.
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